Figure 3: Real-time RT-PCR analysis of MCIP1.4 mRNA. (A) and (B) are amplification plots of MCIP1.4 mRNA from 1 day and 7 day (control and ST) samples, respectively. Controls shown in green and ST shown in red. (C) Quantification of MCIP1.4 mRNA levels relative to 18S using the Delta Delta Ct (2-ΔΔCt) method. Values are represented as mean fold change ± SEM (n=5 - 6 rats per group). * denotes statistical difference between the two groups (1 day ST, p<0.05; 7 days ST, p<0.01).