| Name of the DNA sample |
Dot blot hybridization |
Conventional
PCR |
Real-time PCR |
| |
|
|
Mean of CT value |
Log of DNA
concentration(ng/µL) |
| Conidial suspension* |
− |
+ |
19.75 |
4.2 |
| Pure culture # |
+ |
+ |
14.00 |
100 |
| Assay for Artificially infected samples |
| Seedling (ISD1-3) |
− |
+ |
22.50 |
0.8 |
| Plant root (IPR1-3) |
− |
+ |
21.75 |
1.5 |
| Plant stem (IPS1-3) |
weak signal |
+ |
19.25 |
6.5 |
| Assay for Agricultural field samples |
| Plant root (APR1-3) |
− |
+ |
27.50 |
0.002 |
| Plant stem (APS1-3) |
− |
+ |
26.75 |
0.021 |
| Rhizosphere soil (ARS1-3) |
− |
+ |
33.25 |
0.0015 |
*-5×106 conidia mL-1, + presence, − absence, #- F. udum 193652 |
