Primer Primer sequence 3̀→5̀ Annealing °C
Real time PCR Chitinase1 Forward TTC GTG GAG TGC ATC GGT 60
Chitinase1 Reverse TAN GCN CNN NCC NGG RTT
Defensin 1 Forward CAA TGT AAC TTA AAG TGC CTA ATT ATG 55
Defensin 1 Reverse CTT ATC AGA TCT CAA TGG AGA AAT C
Defnsin 2 Forward TCA CCA AAC TAT TGG ATT TCA A 57
Defensin 2 Reverse GAC TCA ATT TTT GAC TTC TTA ATCC
Differential PCR EZ GCATCACAGACCTGTTATTGCCTC 45
Chi15 GGYGGYTGGAATGARGG 55
EzA1A13 CAG GCC CTT CCA GCA CCCAC 53
Chi 25 GAYTTRTGGGAATAY CC 52
PR1 CCCCAAGCTTTTAGTATGGCTTCTCGTTCACATAAT 40
PR2 CGCGGATCCTACTGGCTATTCTCGATTTTTAATCG 40
Table 1: Primers used in this study and their annealing temperature.