Figure 4: Particle bombardment of coat protein into epidermal cells. Coat protein was cloned in fusion to green fluorescent protein into pRT101- transient expression plasmid under control of CaMV 35S promoter and introduced into epidermal cells by biolistic means (A). Coat protein was extracted from bombarded leaves and detected using TMV-specific antiserum (B). M. Prestained Protein Marker, Broad Range (New England BioLabs, UK), 1. wild-type tobacco, 2. CP-GFP from bombarded leaves.