Figure 3: Influence of atorvastatin, simvastatin and ezetimibe on expressions of miR-221, miR-222 and miR-1303 in HUVEC. Values are shown as mean and SEM and were compared with the vehicle control stimulated with TNFa (0 tnf; dotted line) by oneway ANOVA followed by Tukey post-test. HUVEC were treated for 24 h and stimulated with human TNFa (10 ng/mL) 6 h before RNA extraction. Data were obtained from 4 independent experiments and mRNA expression was calculated using the comparative Ct method using the RNU24 as endogenous reference.