Figure 3: The human Olfactory mucosa (OM) explants were placed in T-25 flask in 1:1 Dulbecco’s modified Eagle’s medium (DMEM)/ Ham’s F12 containing penicillin, streptomycin, gentamicin, bovine insulin, Human epidermal growth factor (hEGF), Transforming Growth Factor-α (TGF-α), cholera toxin, hydrocortisone, Non-essential amino acids (NEAAs), 10% FCS and 10 mM HEPES in a humidified 5% CO2 incubator at 37°C. Spherical clusters were observed on day 5 (A), a mixed population of cells emanating around the explants from the undisturbed flask on day 9 (B) and the attached explants allowed abundant emergence of dense cell population radiating all around with axonal junctions in sparse populated area with occasional presence of olfactory neuronal network is also marked on day 21 (C). The cells were photographed through Nikon Eclipse Ti Phase Contrast with magnification 10X.