Figure 13: Differentiation of the anchored hiPSCs towards cardiomyocytes was demonstrated by qRTPCR. The human induced pluripotent stem cells (hiPSCs) anchored onto the sarcomeres with the heterospecific, tetravalent antibodies (htAbs) targeting SSEA-4, TRA-1-60, myosin, actinin were induced into myogenesis. Expression of the OCT4 and NANOG genes for the transcription factors involved in pluripotency were down regulated over the two week period. Expression of the GATA4 and MEF-2c genes involved in myogenesis were up regulated over the same period of time. The statistical significance was accepted for p < 0.0001.