Method:
SNP Dosage:
Genes:
Differences mRNA expression Function
Array qRT-PCR
1.5 mM 1.5 mM 10 ┬ÁM
Sequestosome 1
Peroxiredoxin 1
Gluthathionereductase
Thioredoxinreductase 1
11.58
2.47
3.37
4.2
2.3
3.87
3.31
6.1
  -1.85
-1.06
1.96
  Antioxidative
protein
ATP binding cassette transporter 3.28 1.35 1.02 Drug resistance
NAD(P)H- dehydrogenase
Glutamate cysteine ligase
Aldehyde oxidase
  5.38
-2.44
5.66
6.84
-3.13
-1.37
-1.72
-2
De-toxification,
cell survival- tumorgenesis
Stress induced phosphoprotein 1 Protein tyrosine phosphatase
ER stress induced domain 1
Valosin containing protein
tRNA guanine transglycosylase
2.52   2.05
2.09
  -1.45
-1.43
1.07
1.61
  -2.63
-2.63
2.17
-1.35
  Repair and removal of damaged proteins
FOS like antigen 1 11.27 6.92 1.22 Modulation of malignant cells
Selected factors regulated by NO in human MSC in comparison to untreated cells showed x-fold differences dependent on the concentration of SNP added. Data were selected from the transcript array data or computed from qRT-PCR as indicated. Factors expressed at higher steady state mRNA levels upon incubation of MSC with SNP are denoted in italics, and factors expressed at lower levels in bold numbers
Table 2: NO-regulated relative mRNA expression of stress response genes in MSC.