Groups Lin-Sca-1+c-kit+CD34- - cells Lin-Sca-1+c-kit+CD34+ - cells Lin-Sca-1+c-kit+ - cells
Lung
Mice received intratrachealNaCl 0.9% 0.07 ± 0.005 0.018 ± 0.001 0.088 ± 0.007
Mice with fibrosis NaCl 0.9% treated 0.12 ± 0.01* 0.06 ± 0.005* 0.18 ± 0.01*
Mice with fibrosis spiperone treated 0.097 ± 0.008* 0.03 ± 0.002* & 0.128 ± 0.011* &
Bone marrow
Intact control 0.39 ± 0.03 0.071 ± 0.006 0.46 ± 0.04
Mice with fibrosis NaCl 0.9% treated 0.35 ± 0.03 0.051 ± 0.005* 0.4 ± 0.03
Mice with fibrosis spiperone treated 0.43 ± 0.04 0.11 ± 0.01* & 0.54 ± 0.05 &
Membrane receptor’s expression of murine HSCs from bone marrow or lung were assayed according to the protocol for BD Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit (BD Biosciences, US). Data represent mean of 3 independent experiments. Results are presented as mean and SEM. * P<0.05 - compared to the mice received intratracheal NaCl 0.9%; & P<0.05 - compared to the mice received intratracheal bleomycin and NaCl 0.9% treated.
Table 4: The content of HSCs and hematopoietic progenitor stem cells in the lung and bone marrow of C57BL/6 mice on the 7th day after bleomycin treatment (M ± m).