Figure 2B: mRNA levels of calponin and SM22αin A7r5 cells, bulk-BMSCs,I-CL cells, and 3T3 cells. Each sample lane was loaded with 20 µg of total RNA, with β-actin expression in each sample used as an intensity control. I-CL cells expressed both calponin and SM22αat the mRNA level; while bulk-stromal cells expressed calponin mRNA at < 1% of the I-CL cell intensity (quantified with a BAS 2500 Bioimage Analyzer). The SMC line A7r5 was used as a positive control.