mRNA
Figure 7: mRNA expression assays. Quantitative PCR was performed to compare the expression of ALP, Sox9 and COMP in undifferentiated (control) and differentiated eBMMSCs from donors 1–5 in presence of PRP. Relative expression of ALP mRNA (A), Sox9 (B) and COMP (C) is shown. The Ct values for each target is normalized with GAPDH mRNA. Fold change in differentiated cells was calculated in comparison to the undifferentiated cells for each target gene. Asterisk demonstrates significant data with p<0.05. Each qPCR was repeated with at least two independent biological samples with n=3-6 for each RNA sample/ target mRNA. Note the significant increase in the expression of each of these targets after differentiation.