Figure 3: Immunohistochemistry of cells supplemented with oxytocin suggested pCPC differentiation into immature cardiomyocytes (A) Immunostaining showed Nkx2.5+ cells but no cardiomyocyte protein (MF-20+) in pCPCs cultured with expansion medium. (B) pCPCs express sarcomeric myosin (MF-20) after 21 days of oxytocin treatment. Scale bars = 500 Ám.