Figure 5: Differentiation of transplanted cells in vivo. A) Cluster of CFDA labeled cells with extensive processes that penetrate into the host neuropil. The linear direction of the needle within the cerebral cortex is evident. Scale bar = 100 Ám. B) Single cell expressing ▀III tubulin is also labeled with the CFDA as shown in (C). Scale bar = 25 Ám for (B) and (C). D) Immunofluorescent staining of CFDA cells with GFAP (arrows). Numerous processes within and around the transplant site are visible. Seven day old transplants. Scale bar = 50 Ám.