Figure 1: Targeted knockdown of brat, numb, or pros in neuroblasts causes overproliferation in the larval brain. Confocal images of larval brains at late third instar stage labeled with mCD8::GFP (green) of wild type (A and E), brat knockdown (B and F), numb knockdown (C and G), and pros knockdown (D and H). Targeted knockdown was carried out by using insc-Gal4 driver (A-D) in all neuroblasts or by wor-Gal4 ase-Gal80 driver in only the type II neuroblast lineages (E-H). Scale bars are 50 Ám.