Figure 1: Human fetal MSC (hfMSC) migrate to Hepatocellular carcinoma (HCC). Migration of hfMSC toward conditioned media from HuH-7 cell line was analyzed using a modified Boyden chamber assay. Briefly, 1x104 MSC were seeded in 8 μm 24-Transwells (BD Biosciences) and analyzed after 8 h. Number of migrating cells was determined by counting the number of propidium iodidestained cells on the underside of the membrane under x200 magnification. Data shown are averages of quadruplicate wells. Bar graph represents percentage of migrated cells normalized to human adult MSC-1 ± SEM.