LV1 GFP+    
  NcoI+MseI     MseI  
Chr Gene Position Chr Gene Position
1 Fcgr4

-

4 Gm11925 (LincRNA)

-

8 Foxl1   10 Trhde

-

1 Usp37 Intron 12 2 B230339M05Rik Intron 22
4 Tardbp Intron 3 12 Prkar2b Intron 2
10 Utrn Intron 23      
19 Nt5c2 Intron 3      

A8

-

-

-

11 Acox1 Intron 3

C12

10 Utrn Intron 23

-

-

-

D1

-

-

-

10 Ppp1r12a Intron 4
F5
3 Tbl1xr1 Intron 10 3 Tbl1xr1 Intron 10

G8

-

-

-

8 Efnb2

-

-

-

-

15 Angpt1

-

Table 2: Identification of vector insertions in transduced GFP+ Sgcb-null cardiac progenitors, and in derived clones. The analysis was performed on the genomic DNA by LM-PCR, after digestions with NcoI+MseI or MseI restriction enzymes. The overall distribution of the vector integration sites in the GFP+ sorted population confirmed the polyclonal profile of lentiviral integrations, while in the GFP+ clones (A8, C12, D1,F5 and G8), obtained by limiting dilutions, only one or two unique insertions were retrieved. Insertion sites detected outside genes are shown in bold, and the closest gene identified is also reported in bold in the table.