integrations

inside gene

outside gene

(NcoI+MseI)

26% (33% intron, 7% exon)

13%

(MseI)

39% (60% intron, 0% exon)

22%

total

65% (93% intron, 7% exon)

35%

Table 3: Genomic distribution of lentiviral integration sites in infected Sgcb-null cardiac progenitors. Percentages of vector integrations obtained by two different digestion protocols (NcoI+MseIand MseI) in infected cardiac progenitors landing inside or outside genes are shown. Note that LV-CMV-eGFP-miR669a2x vector preferentially integrated inside genes as expected.