Figure 3: Lack of effect of estrogen addition on expression of SLPI in BeWo cells. A & B: Effect of Aromatase inhibitor (fadrazole) addition on progesterone (12 h) Induced SLPI expression. C & D: Effect of different concentrations of E2 on expression of SLPI mRNA. A) Cells were incubated with P4 (1M) or P4 (1M) + Fadrazole (1M) or with vehicle (ethanol) control for 12 h. RNA was reverse transcribed and cDNA was subjected to semi-quantitative PCR in the linear range of amplification with GAPDH as an internal control. B) Graphical representation of results presented in A. C) Cells were incubated with different concentrations of E2 ranging from (0-10M) for 12 h. RNA was reverse transcribed and cDNA subjected to semi-quantitative PCR in the linear range of amplification with GAPDH as an internal control. D) Graphical representation of results presented in C, (values expressed as fold increase over control mean S.E. from three independent experiments).