| |
Median staining intensity (score) |
| |
PKC a |
a1-Na,K-ATPase |
| |
sham |
Aldo |
sham |
Aldo |
| Cortex |
|
|
|
|
| Glomerulus |
3 |
4 |
0 |
0 |
| PCT |
1 |
3 |
2 |
3 |
| DCT |
1 |
0 |
4 |
4 |
| CCD |
2 |
0 |
4 |
4 |
| Pcap |
1 |
2 |
1 |
1 |
| Outer medulla |
|
|
|
|
| Outer stripe |
|
|
|
|
| TALH |
3 |
4 |
4 |
4 |
| MCD |
3 |
4 |
4 |
4 |
| PTs |
1 |
2 |
3 |
2 |
| Inner stripe |
|
|
|
|
| TALH |
1 |
2 |
4 |
4 |
| MCD |
1 |
3 |
2 |
3 |
| VR |
3 |
4 |
1 |
1 |
| tLH |
3 |
4 |
1 |
1 |
| Inner medulla |
|
|
|
|
| MCD |
4 |
2 |
3 |
4 |
| VR |
4 |
4 |
1 |
1 |
| tLH |
3 |
4 |
1 |
1 |
Staining intensity: 0: negative, no reactivity; 1: trace, faint, or pale brown staining
with less membrane reactivity; 2: weak, light brown staining with incomplete
membrane reactivity; 3: moderate, shaded of brown staining of intermediate
darkness with usually almost complete membrane reactivity; 4: strong, dark brown
to black staining with usually complete membrane pattern, producing a thick outline
of the cell [10].
PCT: Proximal Convoluted Tubule; DCT: Distal Convoluted Tubule; CCD: Cortical
Collecting Duct; Pcap: Peritubular capillary; TALH: Thick Ascending Limb of
Henle’s Loop; MCD: Medullary Collecting Duct; PTs: Proximal Straight Tubule;
VR=Vasa Recta; tLH: thin limb of Henle’s loop. |
