Figure 6: ERE-luciferase induction in human endometrial stromal cells. Endometrial stromal cells were transiently transfected with ERE-luciferase and ERα and treated with pure compounds E2 (1 μM), genistein (5 μM), and kaempferol (20 μM) with and without the ER antagonist DMA (100 nM) for 48 hours. Data represent mean fold change ± SEM of relative light units normalized to β-gal in triplicate experiments. (a) indicates significant luciferase induction compared to basal DMSO; (b) indicates significant reduction of luciferase induction by DMA as determined by Student’s t-Test, n=3, *p < 0.05.