Figure 1b: Slc2a1 gene expression is not regulated in the pituitary gland of mice. C57BL/6J female mice at different stages of the estrous cycle (diestrus, proestrus, and estrus) were identified by vaginal smears, and then pituitary glands were isolated to prepare RNA for real-time PCR to examine changes in Slc2a1 and Lhb gene expression. Fold effects were determined using pre-pubertal mice as the control. Pre-pubertal females (white bars, n=9-12), and adult females in diestrus (black bars, n=7), proestrus (gray bars, n=6-7), and estrus (striped bars, n=8). Significance was determined by ANOVA as shown by * indicating P<0.05.