Figure 2: The preparative HPLC chromatograms of total steroid saponins and fraction I. Conditions: The GRACE Adsorbosphere C18 column (250 mm x 22 mm, 10 μm); Flow rate: 16 ml min-1; Injection volume: 2 ml; Model 500 UV detector wavelength monitoring: 203 nm. (a): Mobile phase: acetonitrile-water (25:75, v/v) in separation total steroid saponins; (b): Mobile phase: acetonitrile-water (15:85, v/v) in separation fraction I.