Figure 2: Testosterone induces influx of extracellular Ca2+.
(A) Cells were pre-incubated with U-73122 (2 ÁM) for 2 mins before stimulation with T (60 nM). (B) Cells were pre-incubated with Ni2+ (5 mM) for 2 mins before the addition of T (60 nM). (C) Cells were pre-incubated with EGTA (2 mM) for 5 mins and then exposed to T (60 nM). No [Ca2+]i spike was observed. Arrows indicate the time of addition of steroids. Buffer was continuously perfused through the chamber during all experiments. Data shown is the average mean curve of 15 cells from 4 separate endothelial cell donors.