Figure 4: Testosterone rapidly induces NF-?B DNA binding activity in endothelial cells.
(A & B) Bovine aortic endothelial cells (BAECs) were transfected with an NF-Bluciferase reporter vector and then stimulated with T (40 nM), DHT (40 nM) or T-BSA (40 nM) for 0, 7.5, 15, and 30 mins. Results are presented as percentage of 0 min values and are the mean SEM of 3 independent experiments. Results are presented as percentage of 0 min values and are the mean SEM of 3 independent experiments. #P < 0.05 vs. vehicle control/BSA. (C) BAECs were transfected with an NF-?B-luciferase reporter vector and then stimulated with T-BSA (40 nM) or BSA (vehicle control) for 7.5 mins in the absence or presence of calcium-containing media. Results are presented as percentage of 0 min values and are the mean SEM of 3 independent experiments. *P < 0.05 vs. BSA; #P < 0.05 vs. T-BSA+calcium.