Primer |
Sequence |
Product size |
Annealing
temperature |
PGK |
For: 5’ TGCCTGTTGACTTTGTCACTGCTG 3’
Rev: 5’ AGCCTCAGCATACTTCTTGCTGCT 3’ |
112 bp |
60° C |
ADPF |
For: 5’ AGCCCCTTACAGGCATAGGT 3’
Rev: 5’ GTGAGATGGCAGAGAACGGT 3’ |
121 bp |
60° C |
LEP |
For: 5’ GTAGGAATCGCAGCGCC 3’
Rev: 3’ GGGCACACGTTGGACATAGA 3’ |
125 bp |
60° C |
PPAR-γ |
For: 5’ CCAGAAAGCCATTCCTTCAC 3’
Rev: 3’ CGGAGCTGATCCCAAAGTT 3’ |
109 bp |
60° C |
SREBF1 |
For: 5’CAAATAGGCCAGGGAAGTCA 3’
Rev: 5’ACGAGCCACCCTTCAGC 3’ |
138 bp |
60° C |
ALP |
For: 5’ CTA TCC TGG CTC CGT GTC C 3’
Rev: 5’ AGC CCA GAG ATG CAA TCG 3’ |
138 bp |
60° C |
COL I |
For: 5’ CAT GTT CAG CTT TGT GGA CC 3’
Rev: 5’ TTC TGT ACG CAG GTG ATT GG 3’ |
107 bp |
60° C |
IBSP |
For: 5’ GGG CAG TAG TGA CTC ATC CG 3’
Rev: 5’ TCA GCC TCA GAG TCT TCA TCT TC 3’ |
90 bp |
60° C |
ON |
For: 5’ GAGAAAGAAGATCCAGGCCC 3’
Rev: 5’ GCCTGTCTCAAACCCCTCC 3’ |
126 bp |
60° C |
