Figure 2: Morphology of rat 3D-liver microtissue at day 4. (A) Single rat 3D-liver microtissue, composed of primary hepatocytes and non-parenchymal cells as provided in a microtiter plate format (1 microtissue/well). Bar=200 µm. Size analysis of several single microtissues: diameter 319 ± 9 µm; volume 0.0194 ± 0.0014 µm3, n=13. Paraffin sections of rat 3D-liver microtissues stained by (B) hemalaun and eosin and (C) anti-CD68 antibody for the identification of Kupffer cells. (D) Native rat liver section stained for CD68. Bar=100 µm.