Gene Primer sequence GAPDH               Forward 5’-ATGACATCAAGAATGGTG-3’             Reverse 5’-CTGTAGCCAAATTCGTTGTC-3’ Collagen type IV               Forward 5’-CTAATCACAAACTGAATGACTTGACTTCA-3’             Reverse 5’-AAATGGCCCGAATGTGCTTA-3’ FN               Forward 5’-TGGAC CAGAGATCTTGGATGTTC-3’             Reverse 5’-CGCCTAAA ACCATGTTCCTCA-3’ Laminin-5               Forward 5’-GGCTCACTCTGTATTGTTGG-3’             Reverse 5’-ACAGAGACTGCTTTGGTGTG-3’

Table 1: Forward and reverse primers used for real-time PCR assay of ECM genes