Figure 5A-C: EB formation in ultra-low attachment plates. EBs were formed in suspension culture overnight after aggregation. day 4 EBs were cultured in ultra-low attachment plates for 10 days and then transferred to gelatin-coated plates and cultured with either SAGM or BEGM. Photographs were taken at 40X magnification using a Leica DMIL microscope (Leica Microsystems GmbH, Germany) and a Zeiss ApoTome (Carl Zeiss Microimaging GmbH, Germany).Photographs were taken with a Zeiss Axiovert 200M microscope and Axiocam MRm and merged using Axiovision 4.6 software.