Figure 4: MSCs inhibit transcriptional activity of NF-kappa B. A. Schematic of Reporter Assay: U937 cells were transfected with NF-kappa B luciferase plasmid and Renilla firefly luciferase and subsequently cultured with and without MSCs for 24 hours. The cells then received LPS (5ng/mL) or PBS (vehicle), and after a four hour incubation, they were collected and NF-kappa B transcription activity was quantified by the Promega Dual Luciferase Assay according to the manufacturer’s protocol (Promega, Madison, WI). B. Reporter Assay: Coculture with MSCs significantly decreased NF-kappa B transcriptional activity compared to the group cultured without MSCs (p<0.05).