Markers Expression Human skin Skin substitute   Epidermal     Keratins 5 and 14 SB SB; SSB Keratins 1 and 10 SSB SSB Involucrin SP; SG SP; SG (Figure 3) Transglutaminase SP; SG SP; SG Loricrin SG SP; SG (Figure 3) Filaggrin SG SP; SG DLK SG SG   Dermo-epidermal     Laminin V BM BM (Figure 4) Collagen IV BM BM; dermis Collagen VII BM BM (Figure 4) Dermal     Collagen I Dermis Dermis (Figure 3) Collagen III Dermis Dermis (Figure 3) Fibronectin Dermis Dermis       SB: stratum basale; SP: stratum spinosum; SG: stratum granulosum; SC: stratum corneum; SSB: stratum suprabasale; BM: basement membrane

Table 2: Expression of epidermal, dermo-epidermal and dermal markers in human skin and in skin substitute produced with the self-assembly approach.