Figure 9: Immunocytochemistry of cells differentiated from spheres derived from cultured HCECs. Cells migrating out from the spheres express alphasmooth muscle actin (α-SMA: a mesenchymal cell marker), neuron-specific enolase (NSE: a differentiated neural cell marker), microtubule-associated protein 2 (MAP2: a differentiated neural cell marker), and nestin (a neural stem cell marker), indicating that the colonies contain differentiated mesenchymal and neuronal cells. There is no staining for cytokeratin 3 (a differentiated epithelial cell marker). Scale bar=100 μm.