Figure 3: Serum anti-PCC and anti-MCC Ab levels produced by fully MHC-matched or fully MHC-mismatched BXSB mice immunized with PCC. BXSB mice were given 4Gy TBI on day 1, followed by twenty-million bone marrow cells (i.v.) together with 2.0 mg anti-CD40L mAb (i.p.). This simple regimen allowed for the induction of stable bone marrow mixed chimerism. Thick bars indicate mean value of Ab levels in chimeric mice group. Thin bars indicate mean value of Ab levels in untreated BXSB mice group. (A) Upper panel; Fully MHC-matched BXSB chimeric mice that received GFP/B6 bone marrow were immunized with PCC using the same protocol as for the B6 and BXSB mice. Serum anti-PCC and anti-MCC Ab levels between chimeric mice and the untreated BXSB mice were compared. ?: anti-PCC Ab levels in chimeric BXSB mouse serum (n=5). ?: anti-PCC Ab levels in non-treated BXSB mouse serum (n-10). Lower panel; ?: anti-MCC Ab levels in chimeric BXSB mouse serum (n=5). ?: anti-MCC Ab levels in BXSB mouse serum (n=10). *: p<0.01 chimeric group vs. non-treated group. (B) Upper panel; Fully MHC-mismatched BXSB chimeric mice that received B10D2 bone marrow were immunized with PCC. Serum anti-PCC and MCC levels were compared with those of untreated BXSB. ?: anti-PCC Ab levels in chimeric BXSB mouse serum (n=10). ?: anti-PCC Ab levels in non-treated BXSB mouse serum (n=10). Lower panel; ?: anti-MCC Ab levels in chimeric BXSB mouse serum (n=10). ?: anti-MCC Ab levels in BXSB mouse serum (n=10). n.s.: no significance.