S.No Virus Gene target Detection limit in ≤Copy numbers*
Individual PCR Multiplex PCR
1. Porcine circo virus 2 ORF1 1.36×102 5.44×102
2. Porcine Parvovirus NS1 1.41×102 5.62×102
3. African swine fever virus VP73 7.05×101 2.82×102
4. Bovine parvo virus ParVgp1 1.49×101 7.27×101
5. Bovine herpes virus UL39 2.66×101 4.44×102
6. Porcine reproductive and respiratory syndrome virus ORF7 1.38×102 5.84×102
7. Classical swine fever virus NS5B 1.44×102 5.70×102
8. Swine vesicular disease virus   6.35×101 5.48×102
9. Bovine Pestivirus 5’UTR 2.12×101 1.36×102
10. Bovine coronavirus NS1 1.37×101 5.96×102
11. Blue tongue Virus NS1 1.06×102 not included
Table 3: Lower limit of detection of synthetic plasmid constructs in individual and multiplex PCRs. *the formula used for converting the DNA quantities into number of copies = (amount in ngx 6.022×1023) / (length in bp×1×109ng/g ×650 g/ mole of bp).