Table 1

Gene^(a)	Primers		Thermal conditions	(No. of cycles)
Gene^(a)	Sequence	Concentration (pmoles/50µl) of PCR mix	Thermal conditions	(No. of cycles)
GH	GH1F: CTCTGCCTGCCCTGGACT GH1R:GGAGGAGCAGAAGGCAACC GH2F:(TCAGCAGAGTCTTCACCAAC GH2R:(CAACAACGCCATCCTCAC	20	94°C/15 min, 95°C/30 s, 59°C/30 s, 72°C/45 s, 72°C/7 min	35
CSN1S1	Casein S1F: 5’TTCTAAAAGTCTCAGAGGCAG3’ S1R 5’GGGTTGATAGCCTTGTATGT3’	50	97°C/2 min, 60°C/45 s, 72°C/2.5 min, 94°C/45 s, 60°C/45 s,72°C/2.5 min, 72°C/10 min	30
MHC Class II DRB	DRB 1: TATCCCGTCTCTGCAGCACATTTC DRB2: TCGCCGCTGCACACTGAAACTCTC	20	94°C/1 min, 60°C/1.5 min, 72°C/2 min	30
PrP	PrP G1: 5’ATGGTGAAAAGCCACATAGGCAGT3) PrP G2: 5’CTATCCTACTATGAGAAAAATGAG3’	20	95°C/5 min, 96°C/2 min, 60°C/2 min, 72°C/3 min, 72°C/7 min	40

^(a)DNA level used in all protocols was constant (93.0 ng/50μl reaction mix)

Table 1: Optimized protocol for amplification of multiple genes in DNA of a reference Saanen goat sperms.