Bacteriological Culture

Deer ID

Pre CCT1

Post CCT2

Feces3

Oronasal3

Tissue4

Tissue Pool5

13

Neg

Neg

Neg

BCG6 (56)M. fortuitum (14) UIM (14, 28, 42, 56, 89)

Neg

NA

35

Neg

Neg

Neg

M. fortuitum (28, 56, 89)M. alvei (14) UIM (42)

Neg

NA

1072

Neg

Neg

Neg

M. fortuitum (179) UIM (28, 42, 56, 179)

Neg

NA

32M

Neg

Neg

Neg

M. fortuitum (14) UIM (14, 28, 56, 89)

Neg

NA

4M7

Neg

ND

Neg

M. fortuitum (14) UIM (14, 28, 42, 56)

M. simiae

Head

6M7

Neg

ND

Neg

M. fortuitum (14) UIM (42)

M. kansasii

Thoracic

1M

Neg

Reactor

Neg

UIM (14, 42, 56, 89)

BCG

Head, Thoracic

14

Neg

Reactor

Neg

M. fortuitum (14)M. alvei (28) UIM (14, 42)

Neg

NA

29

Neg

Reactor

Neg

M. fortuitum (89) UIM (28, 42)

Neg

NA

20M

Neg

Reactor

Neg

M. fortuitum (14) UIM (14, 28, 42, 56, 89, 179)

Neg

NA

1Comparative cervical tuberculin skin test (CCT) prior to comingling. Neg = no evidence of prior exposure to M. bovis.
2CCT after 180 days of comingling.
3Feces and oronasal swab samples collected prior to comingling and on days 14, 28, 42, 56, 89 and 179 days of comingling. Isolate (sampling day after comingling).
4Isolation of mycobacteria from tissues collected at necropsy after 180 days of comingling. Neg = no mycobacteria isolated.
5For bacteriological isolation of mycobacteria, tissues were allocated into one of three pools; head (mandibular, parotid and medial retropharyngeal lymph nodes), thoracic
(lung, mediastinal and tracheobronchial lymph nodes), or abdominal (mesenteric and hepatic lymph nodes).
6Unspeciated mycobacteria later identifi ed by PCR as M. bovis BCG Danish.
7Deer died due to trauma unrelated to the experimental protocol prior to end of study.
UIM = Unspeciated mycobacteria not found not to be M. bovis BCG Danish by PCR.
NA = not applicable.
ND = not done
.
Table 1: Summary of intradermal tuberculin test and bacteriological culture results from unvaccinated deer co-mingled with Mycobacterium bovis BCG Danish vaccinated deer for 180 days.