Figure 3: (A) Immunohistochemical staining (IHC) assay to compare relative staining intensities of different cancer cell lines probed with RP215 Mab or Ab3 sera. From left to right and top to bottom: Panels A, B and C are IHC staining of OC-3-VGH cells with RP215 (5g/mL), Ab3 sera (1:500 dilution) and normal mouse serum, respectively. Panel D, E and F are those of C-33A with RP215, Ab3 sera and normal mouse serum, respectively. Panel G, H and I are those of A549 cells with RP215, Ab3 sera and normal mouse serum, respectively. Panels J, K and L are those of SKOV-3 cells with RP215, Ab3 sera and normal mouse serum, respectively. (B) Percent increases in apoptosis of cancer cells after incubation with RP215 (10g/mL) and Ab3 sera (1:500 initial dilution) on TUNEL assay. Lanes I, II and III are percentage increases in OC-3-VGH cancer cell apoptosis after 24 hr incubation with RP215, Ab3 sera and normal mouse serum of the same dilution (negative control), respectively. Lane IV and V are C-33A cancer cell apoptosis after 72 hr incubation with Ab3 and normal mouse serum of the same dilution, respectively. Each column represents the mean values S.D. of 4 determinations; and * were relative to negative controls with p < 0.05.