Figure 4: Parasitemia in mice immunized with rTcSP2-CHP or rTcSP2- ATP. BALB/c mice were immunized i.p. with either rTcSP2-CHP or rTcSP2- ATP without adjuvant, and control mice received only PBS. Two weeks after the last immunization, four mice per group were challenged with blood trypomastigotes, and parasitemia levels were evaluated every three days. The values plotted are the mean ± standard deviation for four mice per group and are representative of two independent experiments. At the peak of infection, parasitemia levels were compared using one-way analysis of variance and Tukey’s honestly significant difference test. A significant difference was detected when we compared rTcSP2-CHP and rTcSP2-ATP vs. PBS (* p<0.05).