Figure 1: Transcription of Cpb1/PO (A) and Cpb1/PO protein expression (B) as detected by RT-PCR and immunoblotting in transfected HEK 293 cells. Cells were transfected with pVax/Cpb1/PO expression plasmids and with the negative controls pUC/Cpb1/PO (Mock) as described in materials and methods. Production of specific mRNA (A) was assessed 48 h later in transfected cells. Total RNA was purified from transfected cells and amplified by RT-PCR with specific primers. Cpb1/PO protein was detected by immunoblotting (B) using an antiserum from Leishmania positive dog, only in cells transfected with pVAX1/Cpb1/PO (lane 1, 2).