Cytokine concentration in the supernatants of stimulated PBMCs is shown. (a) IL-2 concentration in the supernatants of PBMC culture 24 h and 48 h after the stimulation; HD (n=9), HER2 0 (n=31), HER2 1+2+ (n=46), HER2 3+ (n=10). Open circles represent each patient’s IL-2 concentration. The solid bar binds the mean values obtained either 24 h or 48 h after the stimulation. Significant differences between HD and HER2 1+2+ 48 h group are shown with ** (p<0.001). (b) IFN-γ: HD (n=4), HER2 0 (n=17), HER2 1+2+ (n=15), HER2 3+ (n=5). (c) IL-4 of the Her2 1+, 2+, or 3+ groups are shown with the healthy donor group HD (n=4), HER2 0 (n=14), HER2 1+2+ (n=10), HER2 3+ (n=3). Solid bars represent the mean value of each group. HD; healthy donors, HER2 0; HER2 expression immunohistochemistry (IHC) score 0 patients. HER2 1+2+; HER2 expression IHC score 1+ and 2+ patients. HER2 3+; HER2 expression IHC score 3+ patients.
