Figure 1: Expression of the P97R1N fusion protein in E. coli JM105 strain by Western blot. Lanes: M, protein ladder; lane 1, purified GST-tagged P97R1N protein; lane 2, purified GST protein; lane 3, purified GST-tagged P97R1 protein. Detection of the P97R1N fusion protein was carried out using anti- GST antibody.