Figure 3: (A) Electrophoresis in a 10% polyacrylamide gel showing (from left to right) the molecular weight marker (“Molecular Weight Marker”, Sigma-Aldrich, Germany), negative control of an immunoprecipitate of cells transfected with the pCID2EtD2prM construct and an immunoprecipitate of cells transfected with the pCID2EtD3prM construct. (B) Imunoblot showing (from left to right) the molecular weight marker (“Molecular Weight Marker”, Sigma-Aldrich, Germany), an immunoprecipitate of cells transfected with the pCID2EtD2prM construct and an immunoprecipitate of cells transfected with the pCID2EtD3prM construct and a negative control of an immunoprecipitate of a cell extract from cells transfected with pCI vector. (C) Result of optical densitometry analysis of E protein expression (western blot densitometry).