Virus Virus titer at 33°С, log10 EID50/ml RCT1       Phenotype
33/37°C 33/38°C 33/39°C 33/40°C 33/25°C
L17-MDV (Н2N2) 9.2 5.0 6.0 7.0 7.5 2.5 ts, ca
NIBRG–23 (H5N1) 6.2 0 0 4,7 6.2 4.0 non–ts, non–ca
1VN/PR/CDC-RG (H5N1) 7.7 1.0 1.0 7.7 7.7 4.0 non-ts, non-ca
t/T/H5N2 LAIV 8.7 4.5 5.7 8.7 8.7 1.5 ts, ca
VN/H5N2 LAIV 9.2 5.0 7.0 9.2 9.2 1.5 ts, ca
VN/H5N1-RG LAIV 8.7 4.2 6.2 8.7 8.7 1.5 ts, ca
1Reproductive capacity of influenza viruses at different temperatures - (ts and ca phenotype) was assessed by titration in eggs at the permissive (33°С), elevated (38, 39, 40°С) and low (25°С) temperatures of incubation and expressed as RCT25,38, 39 or 40= log10 EID50/ml at 33°С –log10 EID50/ml at 25, 38, 39 or 40°С. Eggs were incubated for 2 days at 33, 38, 39 and 40°C and for 6 days at 25°C.
Table 4: Reproductive capacity of reassortant H5N2 LAIV candidates and parental viruses at different temperatures of incubation in embryonated chicken eggs.