Figure 2: Vaccination with double peptide mixture didn’t improve antibody production and protection against disseminated candidiasis in mice. By the dendritic cell (DC)-based approach, the peptide Fba was mixed with peptide Met6 to form double-peptide mixture for immunization in BALB/c mice; peptide mixture induced protection was compared with each individual peptide vaccine. (A) Immunized groups that received the peptide Fba, peptide Met6 or Fba/Met6 mixture vaccines showed 60-80% survival throughout the 50 days post challenge observation period and survived significantly longer as compared to DPBS or DC only controls following the lethal challenge. Peptide Fba induced best protection. (B) Peptide or peptide mixture immunized groups had greatly reduced or even non-detectable viable fungal CFUs in kidneys as compared to control animals (p< 0.0001). However, Fba/ Met6 mixture group didn’t show significantly reduced CFUs in kidneys as compared to individual peptide groups. There is no significant difference in peptide-related antibody responses between Fba/Met6 mixture groups and each individual peptide group (data not shown).