Figure 4: Time between symptoms onset and death. Mice immunized with either 25 μg of r56Lc-1 emulsion+CpG or adjuvant only subcutaneously 3 times at 4 week intervals. The mice were rested for 4 weeks then fed on by either infected or control chiggers. The time between symptoms onset and death are shown as representative data from 3 individual experiments of 40 mice per group. Error bars represent standard errors of the mean (SEM). Vaccine infected=immunized then infected with Lc-1 chiggers; Adjuvant Infected=immunized with adjuvant then infected with Lc-1 chiggers.