MHC-I binding H-2 allele Percentile rank Binding ability Sequence
LLO91-99 H-2-Kb
H-2-Kd
H-2-Ld
67.5
0.7
38.3
UB
GB
UB
Complete
Complete
Complete
LLO296-304 H-2Kb
H-2Kd
H-2Ld
0.2
32
12.5
GB
UB
UB
Complete
Complete
Complete
GAPDH1-22 H-2-Kb
H-2-Kb
H-2-Kd
H-2Kd
H-2-Ld
H-2Ld
1.6
3.3
4.7
5.1
2.3
3.4
GB
WB
WB
WB
IB
WB
5-15*
5-13*
8-16
2-13
8-18
1-10
GAPDH1-15 H-2-Kb
H-2Kd
H-2Ld
3.3
5.1
3.4
WB
WB
WB
5-13*
2-13
1-10
MHC-II binding H-2 allele Bindingthreshold Binding ability Sequence
LLO190-201 H-2-IAb
H-2-IAd
75.1*
>15000
WB
UB
Complete
LLO189-200 H-2-IAb
H-2-IAd
85
11364
WB
UB
Complete
LLO189-201 H-2-IAb
H-2-IAd
51.3
5326
WB
WB-UB
Complete
Complete
GAPDH1-22 H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAb
H-2-IAd
69.15*
70.23
70.91
71.11
74.95*
75.61
3840
WB
WB
WB
WB
WB
WB
WB-UB
4-18*
5-19
2-16
3-17
8-22*
7-21
2-16
GAPDH1-15 H-2-IAb
H-2-IAd
75.72
3240
WB
WB-UB
Complete
Complete
Table 2: H-2 binding force predictions for LLO and GAPDH epitopes. Predictions of binding of peptides to MHC molecules performed with the IEDB analysis source Consensus tool. The lower the percentile ranks obtained, the better the binders. GAPDH1–22 peptide was compared to the predictions of LLO91–99 binding to MHC-I molecules and LLO189–201 binding to MHC-II molecules. *Comparative peptide sequence with similar binding percentiles as LLO peptides. Binding abilities: GB, good binder; WB, weak binder; UB, unable to bind. H-2 binders percentile ranks, <1.0: GB, <2.5: IB, <5: WB and >10:UB (correspond to IC values of <50 nM: high affinity, <500: intermediate affinity, <5000: low affinity). MHC-II binding thresholds, <50: GB, <500: WB, >5000: UB.