flow cytometric
Figure 2: Analysis of IFN-γ secretion by ELISA. IFN-γ secretion levels were measured by ELISA in supernatants of peripheral blood mononuclear cells (PBMCs). (A) Stimulation with antigenic mixture (grey bars) led to significant increase in IFN-γ secretion levels compared with untreated controls, in both groups, before and after immunization. (B) IFN-γ net secretion levels of pre/post vaccination were calculated as the secreted IFN-γ concentration levels in supernatants of antigen-stimulated cultures subtracted by those in the negative control cultures. Vaccination induced a significant increase in secretion levels in the rheumatoid arthritis patients but not in the healthy control group (columns, mean; bars, SD, *p ≤ 0.05, **p ≤ 0.01). RA: Rheumatoid Arthritis.