flow cytometric
Figure 3: Granzyme B activity assay. Ganzyme B activity assays were measured in cultures of peripheral blood mononuclear cells (PBMCs) using a colorimetric substrate. A. The Granzyme B standard curve was plotted as described in the Materials and Method section. B. Stimulation with antigenic mixture in both groups led to an increase in Granzyme B activity compared with untreated controls 4 weeks post-vaccination. C. Comparison of preand post-vaccination net secretion levels (defined as the Granzyme B activity units/mg protein in the stimulated cultures subtracted by those in the negative control cultures) revealed a significant increase in Granzyme B activity in both rheumatoid arthritis patients and healthy controls (columns, mean; bars, standard deviation; *p ≤ 0.05, **p ≤ 0.01, ***p=0.068). RA: Rheumatoid Arthritis.