Healthy

COPD

p value

mRNA

Fold change; median[range]

IFNβ

10.78 [0.24-57.1]

4.8 [0.01-37.4]

0.33

MxA

10.39 [0.33-83.9]

31.25 [0.92-70.0]

0.22

OAS

16.17 [0.35-58.1]

24.85 [4.45-49.01]

0.37

IL-12p35

3.28 [0.29-14.98]

4.98 [0.2-10.16]

0.79

Protein

Concentration (pg/ml); median[range]

IFNα

351.6 [22-660]

81 [50-1880]

0.24

IP-10

38,016 [0-103020]

124,741 [2570-152540]

0.04

PBMC were stimulated in vitro with influenza vaccine. mRNA and protein levels were measured by qPCR and ELISA, respectively. Fold change was calculated as a ratio to baseline (unstimulated PBMCs) whereas concentration refers only to protein levels in supernatants stimulated with influenza vaccine. IFN-α and IP-10 were barely detectable or undetectable in unstimulated cultures. Results are shown as median ± IQ; n = 6 COPD subjects and 10 healthy controls.

Table 3: Innate immune function in healthy subjects and COPD patients.