Figure 2: Induction of CLU protein in both tubules and glomeruli following transplant rejection or IRI. Kidney tissues were fixed in 10% buffered formaldehyde, embedded in paraffin, and then sectioned for immunohistochemical staining with goat polyclonal anti-CLU-α (C-18). (A) Biopsy sample of rejecting kidney transplant (Banff 97 grade 1 chronic rejection) from a 15-year old female patient (provided by Dr. Alex Magil, St. Paul’s Hospital, Vancouver, BC, Canada). (B) Mouse kidneys with renal ischemia-reperfusion injury. Brown color (also pointed by red arrows): positively stained cells. The data are presented as a typical image from each examination.