cAMP formation ( % of forskolin stimulated cAMP levels)
L-AP4 [10µM]
LY341495 [10µM]
LY341495 [10µM] / L-AP4 [100µM]
Induced
Uninduced
Induced
Uninduced
Induced
Uninduced
Wild type
54 ± 3 (7)
77 ± 7 (2)
246 ± 6 (4)
106 ± 8 (3)
47 ± 1 (3)
90 ± 1 (3)
Double cysteine mutants
C754A
38 ± 1 (5)
91 ± 4 (3)
324 ± 13 (3)
101 ± 3 (3)
34 ± 1 (3)
79 ± 1 (3)
C765A
37 ± 2 (5)
95 ± 3 (3)
205 ± 4 (3)
99 ± 2 (3)
36 ± 2 (3)
88 ± 4 (3)
C793A
107 ± 6 (5)
103 ± 3 (3)
185 ± 7 (3)
104 ± 3 (3)
79 ± 1 (3)
88 ± 3 (3)
Single cysteine mutants
C754A/C765A
52 ± 5 (6)
83 ± 3 (8)
199 ± 23 (6)
102 ± 2 (6)
50 ± 3 (6)
91 ± 3 (6)
C765A/C793A
108 ± 6 (2)
83 ± 3 (3)
236 ± 3 (3)
112 ± 7 (3)
88 ± 3 (3)
91 ± 1 (3)
C754A/C793A
80± 3 (4)
98 ± 3 (3)
201 ± 8 (3)
104 ± 4 (3)
82 ± 3 (3)
101 ± 6 (3)
Cys-less mutant
C754A/C765A/C793A
79 ± 3 (5)
87 ± 5 (4)
275 ± 12 (3)
91 ± 4 (3)
70 ± 1 (3)
84 ± 5 (3)
Table 1: cAMP levels in stable cell lines of wild-type and cysteine mutants of mGluR6 in presence of L-AP4 and LY341495. Data is represented as mean ± SEM (number
of independent experiments).