Receptor construct

cAMP formation ( % of forskolin stimulated  cAMP levels)

L-AP4 [10µM]

LY341495 [10µM]

LY341495 [10µM] / L-AP4 [100µM]

 

Induced

Uninduced

Induced

Uninduced

Induced

Uninduced

Wild type

54 ± 3 (7)

77 ± 7 (2)

246 ± 6 (4)

106 ± 8 (3)

47 ± 1 (3)

90 ± 1 (3)

Double cysteine mutants

           

C754A

38 ± 1 (5)

91 ± 4 (3)

324 ± 13 (3)

101 ± 3 (3)

34 ± 1 (3)

79 ± 1 (3)

C765A

37 ± 2 (5)

95 ± 3 (3)

205 ± 4 (3)

99 ± 2 (3)

36 ± 2 (3)

88 ± 4 (3)

C793A

107 ± 6 (5)

103 ± 3 (3)

185 ± 7 (3)

104 ± 3 (3)

79 ± 1 (3)

88 ± 3 (3)

Single cysteine mutants

           

C754A/C765A

52 ± 5 (6)

83 ± 3 (8)

199 ± 23 (6)

102 ± 2 (6)

50 ± 3 (6)

91 ± 3 (6)

C765A/C793A

108 ± 6 (2)

83 ± 3 (3)

236 ± 3 (3)

112 ± 7 (3)

88 ± 3 (3)

91 ± 1 (3)

C754A/C793A

80± 3 (4)

98 ± 3 (3)

201 ± 8 (3)

104 ± 4 (3)

82 ± 3 (3)

101 ± 6 (3)

Cys-less mutant

           

C754A/C765A/C793A

79 ± 3 (5)

87 ± 5 (4)

275 ± 12 (3)

91 ± 4 (3)

70 ± 1 (3)

84 ± 5 (3)

Table 1: cAMP levels in stable cell lines of wild-type and cysteine mutants of mGluR6 in presence of L-AP4 and LY341495. Data is represented as mean ± SEM (number of independent experiments).